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KMID : 0364819910290020143
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Korean Journal of Microbiology
1991 Volume.29 No. 2 p.143 ~ p.147
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Characteristics of Bradyrhizobium japonicum SNU001, a Symbiotic Strain of Glycine max
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Abstract
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1
The root nodules and Glycine max were classified as determinate nodule based on their morphological characteristics, and isolated endosymbiont as a Bradyrhizobium based on its growth rate and single subpolar flagellum. The isolate was similar to B. japonicum USDA110 in utilization of carbon source, growth at 38°C and 2% NaCl, production of H2S and especially in the restriction endonuclease digestion pattern of symbiotic genes, allowing them to be´placed in sTI group together. The former, however, grew better than the later in broad pH range from 5.0 to 9.5. Infectivity and effectivity of the isolate were confirmed by inoculation of soybean seedlings with the isolates. Characteristics of the reisolated endosymbiont from induced root nodules were identical to those of the first isolate. From these results, it was confirmed that Bradyrhizobium strain isolated from the root nodules of Glycine mar was a real symbiont, and was named B. japonicum SNU001.
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